abstract：:Diquat is a hepatotoxin whose toxicity in vivo and in vitro is mediated by redox cycling and greatly enhanced by pretreatment with 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), an inhibitor of glutathione reductase. The mechanism by which redox cycling mediates diquat cytotoxicity is unclear, however. Here, we have att...

journal_title：Archives of biochemistry and biophysics

authors： Sandy MS,Moldeus P,Ross D,Smith MT

更新日期：1987-11-15 00:00:00

abstract：:Deoxycholic acid, a colon tumor promoter, was found to bind covalently to DNA and RNA in the presence of methyl linoleate hydroperoxide and ferrous ion. This binding was shown to occur specifically with guanine residues and its covalent nature was demonstrated by analysis of hydrolysates of nucleic acid adducts. These...

journal_title：Archives of biochemistry and biophysics

authors： Kodama M,Inoue F,Nakayama T,Nagata C

更新日期：1987-11-01 00:00:00

abstract：:The subcellular mechanism of alkenyl halide S-conjugate-induced nephrotoxicity was studied in mitochondria isolated from rat kidney cortex in vitro using the cysteine conjugate of hexachloro-1,3-butadiene, i.e., S-pentachlorobutadienyl-L-cysteine (PCBC) as a model substrate. Respiring mitochondria exposed to various c...

journal_title：Archives of biochemistry and biophysics

authors： Wallin A,Jones TW,Vercesi AE,Cotgreave I,Ormstad K,Orrenius S

更新日期：1987-11-01 00:00:00

abstract：:In the present study we show that monospecific antibody against cytochrome P-450a completely inhibits testosterone 7 alpha-hydroxylation in hepatic microsomes of untreated male or female rats or rats of either sex treated with dexamethasone. These data are in contrast with those of K. Nagata et al. (1987, J. Biol. Che...

journal_title：Archives of biochemistry and biophysics

authors： Levin W,Thomas PE,Ryan DE,Wood AW

更新日期：1987-11-01 00:00:00

abstract：:Purification of the scrapie agent by methods using digestion with proteinase K yields a protein product, PrP-27-30, with an apparent mass of 27-30 kDa (D. C. Bolton et al. (1982) Science 218, 1309-1311; S. B. Prusiner et al. (1982) Biochemistry 21, 6942-6950). In contrast, a 33-37 kDa glycoprotein, HaSp33-37, was the ...

journal_title：Archives of biochemistry and biophysics

authors： Bolton DC,Bendheim PE,Marmorstein AD,Potempska A

更新日期：1987-11-01 00:00:00

abstract：:Western blots using a polyclonal and a monoclonal antibody raised against rat liver cytochrome P-450b indicate tissue-specific expression of low levels of cytochrome P-450's b and e. P-450b and P-450e were expressed very selectively in, respectively, lung and adrenal microsomes of untreated rats but neither isozyme wa...

journal_title：Archives of biochemistry and biophysics

authors： Christou M,Wilson NM,Jefcoate CR

更新日期：1987-11-01 00:00:00

abstract：:Neutral and acidic glycolipids were extracted from chicken hearts. The neutral and acidic compounds were separated by preparative thin-layer chromatography into eight and two fractions, respectively. Total hydrolysis by mineral acid, permethylation analysis, and sequential cleavage with exoglycosidases showed the pres...

journal_title：Archives of biochemistry and biophysics

authors： Jennemann R,Felding-Habermann B,Geyer R,Stirm S,Wiegandt H

更新日期：1987-10-01 00:00:00

abstract：:Thermal perturbation techniques have been used to probe structural features of the nicotinic acetylcholine receptor (AcChR). The information obtained from differential scanning calorimetry (DSC) of AcChR membranes (M.C. Farach and M. Martinez-Carrion (1983) J. Biol. Chem. 258, 4176) in the absence and in the presence ...

journal_title：Archives of biochemistry and biophysics

authors： Artigues A,Villar MT,Ferragut JA,Gonzalez-Ros JM

更新日期：1987-10-01 00:00:00

abstract：:Human seminal fluid was recently found to contain 18,19-dehydroprostaglandins E1 and E2 (E. H. Oliw, H. Sprecher, and M. Hamberg, (1986) J. Biol. Chem. 261, 2675-2683). In the present study, the cis and trans isomers of 18,19-dehydroprostaglandins E1 and E2 were prepared by incubation of microsomes of ram vesicular gl...

journal_title：Archives of biochemistry and biophysics

authors： Oliw EH,Benthin G,Hamberg M

更新日期：1987-10-01 00:00:00

abstract：:Pyruvate carboxylase deficiency was previously reported to be the biochemical lesion in a yeast mutant, designated pyc, which cannot utilize ethanol, acetate, pyruvate, aspartate, or oxaloacetate as the sole carbon source [C. Wills and T. Melham (1985) Arch. Biochem. Biophys. 236, 782-791; C. Wills et al. (1986) Arch....

journal_title：Archives of biochemistry and biophysics

authors： Lim F,Rohde M,Morris CP,Wallace JC

更新日期：1987-10-01 00:00:00

abstract：:The presence of multiple proteases in the culture filtrate of Streptomyces moderatus was detected. After preliminary purification by ammonium sulfate precipitation and decolorization using DEAE-cellulose, the fractionation of various proteases was carried out using CM-trisacryl cation-exchange chromatography. By this ...

journal_title：Archives of biochemistry and biophysics

authors： Chandrasekaran S,Dhar SC

更新日期：1987-09-01 00:00:00

abstract：:Glycosphingolipids were extracted from human semen and purified. Based on the fluorometric assay of sphingosine, in spermatozoa a content of 4.4 +/- 0.9 nmol/10(8) cells of gangliosides and 22.1 +/- 1.7 nmol/10(8) cells of neutral glycosphingolipids was determined. Seminal plasma contained 4.1 +/- 0.6 nmol ganglioside...

journal_title：Archives of biochemistry and biophysics

authors： Ritter G,Krause W,Geyer R,Stirm S,Wiegandt H

更新日期：1987-09-01 00:00:00

abstract：:We propose that adverse effects of the antibiotic metronidazole may be due, wholly or in part, to its conversion to a thiamine analog and consequent vitamin B1 antagonism. Consistent with this hypothesis, the drug is accepted as a substrate for the thiaminase (EC 2.5.1.2) elaborated as an exoenzyme by the human gut fl...

journal_title：Archives of biochemistry and biophysics

authors： Alston TA,Abeles RH

更新日期：1987-09-01 00:00:00

abstract：:The purpose of the current study is to present further evidence for prothrombin self-association as assessed by chemical crosslinking. When the self-association (evaluated by covalent crosslinking with dithiobis(succinimidylpropionate) of prothrombin or fragment 1 was evaluated at the same molar concentration of prote...

journal_title：Archives of biochemistry and biophysics

authors： Tarvers RC,Roberts HR,Straight DL,Featherstone GL,Lundblad RL

更新日期：1987-09-01 00:00:00

abstract：:The binding of Toxin A isolated from Clostridium difficile to rabbit erythrocyte glycolipids has been studied. Total lipid extracts from rabbit erythrocytes were subjected to thin-layer chromatography and toxin-binding glycolipids detected by using 125I-labeled Toxin A in a direct binding overlay technique. Two major ...

journal_title：Archives of biochemistry and biophysics

authors： Clark GF,Krivan HC,Wilkins TD,Smith DF

更新日期：1987-08-15 00:00:00

abstract：:The crystalline beef liver protein of Sumner and Dounce (A. L. Dounce, P. Z. Allen, and G. A. Mourtzikos (1978) Arch. Biochem. Biophys. 188, 251-265) termed FTBL (football) protein because of the shape of its crystals, has been identified as a crystalline leucine aminopeptidase (LAP), on the basis of its high specific...

journal_title：Archives of biochemistry and biophysics

authors： Dounce AL,Allen PZ

更新日期：1987-08-15 00:00:00

abstract：:To elucidate the physiological significance of ketone bodies on insulin and glucagon secretion, the direct effects of beta-hydroxybutyrate (BOHB) and acetoacetate (AcAc) infusion on insulin and glucagon release from perfused rat pancreas were investigated. The BOHB or AcAc was administered at concentrations of 10, 1, ...

journal_title：Archives of biochemistry and biophysics

authors： Ikeda T,Yoshida T,Ito Y,Murakami I,Mokuda O,Tominaga M,Mashiba H

更新日期：1987-08-15 00:00:00

abstract：:Lysosomes were isolated from Chinese hamster ovary cells by fractionation of a postnuclear supernatant in consecutive density gradients. By marker enzyme analysis, the preparation was 63-fold enriched for lysosomes compared to the homogenate and contained at most trace amounts of marker activities for plasma membrane,...

journal_title：Archives of biochemistry and biophysics

authors： Madden EA,Wirt JB,Storrie B

更新日期：1987-08-15 00:00:00

abstract：:The mechanism by which chemical inducers of the stress response inhibit protein synthesis was examined. All the chemicals tested principally inhibit the initiation phase of translation. Covalent modification of the initiation factor proteins does not constitute a common mechanism. Eukaryotic initiation factor (eIF)-2 ...

journal_title：Archives of biochemistry and biophysics

authors： Duncan RF,Hershey JW

更新日期：1987-08-01 00:00:00

abstract：:The antioxidant, butylated hydroxytoluene (BHT), causes lung toxicity in mice followed by regenerative repair, and can also modulate the development of carcinogen-induced lung adenomas. We are investigating changes in pulmonary biochemistry following BHT treatment in order to understand the mechanisms of BHT-induced p...

journal_title：Archives of biochemistry and biophysics

authors： Blumenthal EJ,Malkinson AM

更新日期：1987-07-01 00:00:00

abstract：:A theoretical and experimental study has been made of the advancing elution profile in frontal gel chromatography of interacting systems for which the elution volume of the complex is smaller than that of the larger reactant. First, Gilbert-Jenkins theory is used to delineate the form of the elution profile from the m...

journal_title：Archives of biochemistry and biophysics

authors： Cann JR,Winzor DJ

更新日期：1987-07-01 00:00:00

abstract：:The cardiomyopathy produced by the widely used anticancer drug adriamycin (ADR) is believed to be related to the production of reaction oxygen species and consumption of reduced glutathione (GSH) during redox cycling of the drug. Protection by vitamin E against the toxicity of ADR was studied in a model of compromised...

journal_title：Archives of biochemistry and biophysics

authors： Pascoe GA,Reed DJ

更新日期：1987-07-01 00:00:00

abstract：:Aryl hydrocarbon hydroxylase activity is most closely associated with cytochrome P-450c in the rat and cytochrome P1-450 in the mouse. The sequence for the orthologous human gene coding for this enzymatic activity has been determined from several sources: cytochrome P-450c isolated from human embryonic DNA [K. Kawajir...

journal_title：Archives of biochemistry and biophysics

authors： Iversen PL,Heiger WJ,Bresnick E,Hines RN

更新日期：1987-07-01 00:00:00

abstract：:The total lipid composition of highly purified plasma membranes from light-grown barley (Hordeum vulgare) leaves was investigated. The plasma membranes were separated from intracellular membranes by subfractionation of the microsomal fraction using aqueous polymer two-phase partitioning. A novel finding was that gluco...

journal_title：Archives of biochemistry and biophysics

authors： Rochester CP,Kjellbom P,Andersson B,Larsson C

更新日期：1987-06-01 00:00:00

abstract：:An N-acetyl-D-galactosamine-specific lectin has been isolated from the two seed forms of the hog peanut (Amphicarpaea bracteata) using an affinity support containing the synthetic type A blood group trisaccharide alpha-D-GalNAc-(1,3)-[alpha-L-Fuc-(1,2)]-beta-D-Gal (Synsorb A). The affinity-purified lectin appears to b...

journal_title：Archives of biochemistry and biophysics

authors： Maliarik MJ,Roberts DD,Goldstein IJ

更新日期：1987-05-15 00:00:00

abstract：:Purified NAD-malic enzyme from Ascaris suum is rapidly inactivated by the arginine reagent, 2,3-butanedione, and this inactivation is facilitated by 30 mM borate. Determination of the inactivation rate as a function of butanedione concentration suggests a second-order process overall, which is first order in butanedio...

journal_title：Archives of biochemistry and biophysics

authors： Rao GS,Kong CT,Benjamin RC,Harris BG,Cook PF

更新日期：1987-05-15 00:00:00

abstract：:We previously reported that rat testis and porcine testis contain a physicochemically indistinguishable Mr 32,000 calmodulin-binding protein, which we named "calspermin." In this study, antiserum against calspermin from porcine testis was raised in a rabbit, and a radioimmunoassay was developed. The antiserum reacted ...

journal_title：Archives of biochemistry and biophysics

authors： Ono T,Koide Y,Arai Y,Yamashita K

更新日期：1987-05-15 00:00:00

abstract：:The polyglutamation kinetics of methotrexate (MTX) in MCF-7 human breast cancer cells have been formulated mathematically. The model takes account of glutamation and hydrolysis kinetics up through the pentaglutamate level, increased synthesis of dihydrofolate reductase following exposure to drug, reversible tight-bind...

journal_title：Archives of biochemistry and biophysics

authors： Morrison PF,Allegra CJ

更新日期：1987-05-01 00:00:00

abstract：:A glucose analog, N-(bromoacetyl)-D-glucosamine (GlcNBrAc), previously used to label the glucose binding sites of rat muscle Type II and bovine brain Type I hexokinases, also inactivates rat brain hexokinase (ATP:D-hexose 6-phosphotransferase, EC 2.7.1.1) with pseudo-first-order kinetics. Inactivation occurs predomina...

journal_title：Archives of biochemistry and biophysics

authors： Schirch DM,Wilson JE

更新日期：1987-05-01 00:00:00

abstract：:The cytochrome P-450-dependent cholesterol side chain cleavage system of the brain has been studied using nonsynaptic mitochondria as the source of enzymatic activity. The system has been found to bind cholesterol and 11-deoxycorticosterone, producing type I difference spectra, whereas the binding of pregnenolone indu...

journal_title：Archives of biochemistry and biophysics

authors： Walther B,Ghersi-Egea JF,Minn A,Siest G

更新日期：1987-05-01 00:00:00

abstract：:The activities, properties, and steady-state kinetics of the five enzymes catalyzing the synthesis of 1-acyl- and 1-alkyl-sn-glycerol 3-phosphate in the cultured skin fibroblasts from Zellweger syndrome patients and normal controls were studied in detail. Judging from their Km and Vmax values, glycerol phosphate acylt...

journal_title：Archives of biochemistry and biophysics

authors： Webber KO,Datta NS,Hajra AK

更新日期：1987-05-01 00:00:00

abstract：:A preliminary study (J.M. Mata, R. Assad, and B. Peterkofsky (1981) Arch. Biochem. Biophys. 206, 93-104) suggested that chick embryo limb bone microsomes took up and concentrated [14C]ascorbate in the presence of cofactors for prolyl hydroxylase. In the present study, we found that the apparent Km for ascorbate in the...

journal_title：Archives of biochemistry and biophysics

authors： Peterkofsky B,Tschank G,Luedke C

更新日期：1987-04-01 00:00:00

abstract：:A new plant lectin from elderberry (Sambucus nigra L.) bark, which was shown by immunochemical techniques to bind specifically to terminal Neu5Ac(alpha 2-6)Gal/GalNAc residues of glycoconjugates, was immobilized onto Sepharose 4B (SNA-Sepharose) and its carbohydrate binding properties was determined using a series of ...

journal_title：Archives of biochemistry and biophysics

authors： Shibuya N,Goldstein IJ,Broekaert WF,Nsimba-Lubaki M,Peeters B,Peumans WJ

更新日期：1987-04-01 00:00:00

abstract：:By anion-exchange chromatography, the nitrogen fixing photosynthetic stem nodules and nonphotosynthetic root nodules of Sesbania rostrata are shown to contain the same seven major components of leghemoglobin (Lb), numbered LbI-LbVII in order of elution, although in different proportions. No novel component was found i...

journal_title：Archives of biochemistry and biophysics

authors： Bogusz D,Kortt AA,Appleby CA

更新日期：1987-04-01 00:00:00

abstract：:It was shown previously that the ATP synthase complex of bovine heart mitochondria contains an essential set of thiols or dithiols in its membrane sector (F0), whose modification by various reagents results in uncoupling [Yagi, T., and Hatefi, Y. (1984) Biochemistry 23, 2449-2455]. The sensitivity to modifiers was inc...

journal_title：Archives of biochemistry and biophysics

authors： Yagi T,Hatefi Y

更新日期：1987-04-01 00:00:00

abstract：:Forskolin, a diterpene that exerts several pharmacological effects, activates adenylate cyclase in brain and in some other mammalian tissues. Properties of forskolin activation of adenylate cyclase from central nervous system of the dipterous Ceratitis capitata are described. The interaction of forskolin with the inse...

journal_title：Archives of biochemistry and biophysics

authors： Guillen A,Haro A,Municio AM

更新日期：1987-04-01 00:00:00

abstract：:Insulin has pleiotropic effects on sensitive cells, including the regulation of specific mRNA accumulation initiated by the binding of insulin to its plasma membrane receptor. Lectins, such as wheat germ agglutinin (WGA) and concanavalin A (Con A), are known to be insulin mimetic. It is thought that WGA and Con A inte...

journal_title：Archives of biochemistry and biophysics

authors： Messina JL,Hamlin J,Larner J

更新日期：1987-04-01 00:00:00

abstract：:Alkaline phosphatase (AP) was purified to over 90% homogeneity from rat osteosarcoma by acetone precipitation followed by chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxyapatite. The purified enzyme had a specific activity of 759 units/mg protein at its optimal pH (10.5), and a Km of 0.8 mM for p-nitroph...

journal_title：Archives of biochemistry and biophysics

authors： Nair BC,Majeska RJ,Rodan GA

更新日期：1987-04-01 00:00:00

abstract：:Electrophoretically homogeneous preparations of catalytic subunit (C) of cAMP-dependent protein kinase isolated according to two different procedures from holoenzyme type I and type II from rabbit and from holoenzyme type II from rat skeletal muscle and from bovine cardiac muscle can be separated on carboxymethyl cell...

journal_title：Archives of biochemistry and biophysics

authors： Kinzel V,Hotz A,König N,Gagelmann M,Pyerin W,Reed J,Kübler D,Hofmann F,Obst C,Gensheimer HP

更新日期：1987-03-01 00:00:00

abstract：:M. Kuwajima, C. B. Newgard, D. W. Foster, and J. D. McGarry (1986, J. Biol. Chem. 261, 8849-8853) have concluded that the reason postprandial hepatic glycogenesis occurs primarily from gluconeogenic precursors rather than glucose is because glucokinase activity is insufficient to support the observed rates of glycogen...

journal_title：Archives of biochemistry and biophysics

authors： Davidson AL,Arion WJ

更新日期：1987-02-15 00:00:00